lnterleukin-4 as a potent down-regulator for human alveolar macrophages capable of producing tumour necrosis factor-a and lnterleukin-1
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چکیده
lnterleukin-4 as a potent down-regulator for human alveolar macrophages capable of producing tumour necrosis factor-a and interleukin-1. S. Sone, H. Yanagawa, Y. Nishioka, E. Orino, G. Bhaskaran, A. Ni~ K. Mizuno, Y. Heike, F. Ogush~ T. Ogura. ABSTRACT: The effects of recomblnant human lnterleukln-4 (IL-4) on the produc· tlon of lnterleuldn·l (IL-l) and tumour necrosis factor-a (fNFa) by human alveolar macrophages (AM) and autologous peripheral blood monocytes (PBM) In response to lipopolysaccharide (LPS) were examined. AM and PBM were obtained by bronchoalveolar lavage and centrifugal elutrlatlon, respectively, from healthy donors. The production ofiL·l (a and~) and TNFa by human AM and PBM were quantitated by enzyme immunoassays (EIA). When activated with LPS, AM secreted much more TNFa, but less IL-l~ than PBM. The production of IL-l (a and ~) by activated AM and autologous PBM was suppressed dose-dependently by IL-4. The Inhibitory effect of IL-4 was greatest when it was added to AM or PBM simultaneously with LPS or within 3 h after LPS. The suppressive effect of IL-4 was completely neutralized by pretreatment with rabbit anti·IL-4 antiserum. IL-4 also suppressed the production ofiL·l and TNFa by monocyte-derived macrophages. As measured by thymocyte eo-stimulation assay, the production of cell-associated IL·l was Inhibited by coculture of AM plus LPS with IL-4. Northern blot analysis showed suppression by IL-4 of expression of messenger ribonucleic acid (mRNA) for IL-l and TNFa In LPS-stimulated AM. We conclude that IL-41s a potent down-regulator for human alveolar macrophages c.apable of producing IL-l and TNFa. Eur Respir J., 1992, 5, 174-181. Third Department of Internal Medicine The University of Tokushima School of Medicine Tokushima Japan.
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تاریخ انتشار 2012